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KMID : 0613820070170030396
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Journal of Life Science
2007 Volume.17 No. 3 p.396 ~ p.401
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The Effects of Diesel Exhaust Particulates and Particulate Matters on the ICAM-1 and VCAM-1 Expression in the Lung of Asthma-incuced Mouse
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Li Tianzhu
Lee Soo-Jin
Jang Yang-Ho
Lee Jung-Hak
Park Se-Jong
Park Jun-Hong
Chang Byung-Joon
Lee Jong-Hwan
Choe Nong-Hoon
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Abstract
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This research investigated whether exposure of diesel exhaust particulate (DEP) and particulate metter (PM) effect on intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in asthma-induced Balb/c and IL-10 knock out (KO) mouse. Mouse was sensitized with intraperitoneal injection with ovalbumin, followed by challenges with intranasal ovalbumin. After induction of asthma mouse placed in the inhalation chamber and exposed to DEP and PM (10 mg/m3). The evidences of pulmonary inflammation were assessed by immunohistochemical stain and westen blot against ICAM-1 and VCAM-1 in the lung tissue. In the immunohistochemical stain, positive reactions for ICAM-1 and VCAM-1 were much stronger in asthma-induced groups and asthma-induced group with DEP or PM than control groups. Although mild positive reactions were appeared in asthma-induced IL-10 KO mice groups, positive reactions were very strong in the asthma-induced group with DEP or PM. In Western blot, expression of VCAM-1 was increased in asthma-induced group with DEP or PM than asthma-induced groups. In the IL-10 KO mouse, ICAM-1 and VCAM-1 expression were increased in asthma-induced group with DEP or PM than asthma-induced groups. DEP and PM exposure have additive effects on the aggravation of inflammatory signs in the asthma-induced murine model. These results suggest that inhalation of DEP and PM in asthmatic patients may aggravate clinical symptoms.
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KEYWORD
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Asthma-induced mouse, IL-10 knock out mouse, ICAM-1, VCAM-1, diesel exhaust particulate and particulate metter
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